Adaptive Immunity

Gene Expression and Transcription

Note Packet

Answers to Objective Statements

Lesson

Topic

Statement(s) and Objective(s)

Activities

1	Central Dogma Overview		Central dogma “where are we?” slide Central dogma model (continues in unit 19) General directions Completed model 11X17 Blank model 11x17 Blank model 8.5X11 Transcription factor video RNA polymerase photo Transcription model photo Spliceosome front Spliceosome back Splicing video Splicing model photo front Splicing model photo back Example of model in action (IG)
2	Transcription	2.7.U4: Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase). Define transcription. Outline the process of transcription, including the role of RNA polymerase and complementary base pairing. Identify the sense and antisense strands of DNA given a diagram of translation. 7.2.A1: The promoter as an example of non-coding DNA with a function.. Outline the role of promoter DNA. 7.2.U4: Transcription occurs in a 5’ to 3’ direction. Describe the initiation of transcription, including the role of the promoter, transcription factors, the TATA box and RNA polymerase. Describe elongation of transcription, including the role of nucleotide triphosphates and the direction of transcription. Describe termination of transcription, including the role of the terminator.	Transcription notes Transcription handwritten Transcription CFU Transcription POGIL Online transcription simulation Scitable: Info in DNA is decoded Scitable: DNA transcription Scitable: RNA polymerase
3	RNA modification	7.2.U5: Eukaryotic cells modify mRNA after transcription. List two major differences in gene expression between prokaryotic cells and eukaryotic cells. Describe the three post-transcriptional modifications of pre-mRNA in eukaryotes.	RNA modification notes RNA Splicing and modifying handwritten notes Playdoh splicing RNA modification CFU Regulation of gene expression table (from unit 17)
4	Alternative Splicing	7.2.U6: Splicing of mRNA increases the number of different proteins an organism can produce. Describe the process of alternative RNA splicing. Outline an example of alternative splicing the results in different protein products.	Antibody Alternative splicing activity Course Source Alt Splicing lesson Pre-activity slides Pre-quiz Day 1 lesson Day 1 slides Day 2 lesson Day 2 slides Post-quiz A&B:Octopus alternative splicing A&B: RNA controls alt splicing A&B: the alternative genome Questions for the alternative genome
5	Wrap Up and Review		Review slides Final knowledge audit (also includes regulation of gene expression, unit 17) 1-pager summary Quizizz game Kahoot game